Ultrahigh throughput screening yields improved DNA polymerase
Loop-mediated isothermal amplification (LAMP), a DNA amplification technique valued for its speed, specificity, sensitivity, and simplicity, is increasingly employed in disease diagnostics and genetically modified organism detection. At the heart of this method lies the Bst DNA polymerase, whose enzymatic properties—such as strand displacement activity, thermostability, and catalytic efficiency—are critical to the success of isothermal amplification, typically conducted at 60–65°C.
Loop-mediated isothermal amplification (LAMP), a DNA amplification technique valued for its speed, specificity, sensitivity, and simplicity, is increasingly employed in disease diagnostics and genetically modified organism detection. At the heart of this method lies the Bst DNA polymerase, whose enzymatic properties—such as strand displacement activity, thermostability, and catalytic efficiency—are critical to the success of isothermal amplification, typically conducted at 60–65°C.
